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What is HIP?

The Human Immunology Portal (HIP) is an online public resource for scientists investigating the human immune system. HIP facilitates the use of human primary tissue to better understand the human immune response to infectious diseases, by providing complete protocols, tips, and other resources for immunologists working within the human system. Our goal is to help researchers more easily translate their research from mouse to human models, and thereby close the gap between basic research and clinical therapies such as new vaccines and drug targets.

Explore HIP resources:


HIPprotocols are standardized, detailed protocols for human sample processing, cell isolation and downstream assays for human immunology researchers. Many of these protocols include step-by-step technical videos to support both researchers entering the human immunology field, as well as veteran researchers who are looking for new tips and tricks to optimize their existing human immunology protocols.


HIPtips are technical tips and additional insights into best practices for human sample processing and immune cell isolation. These tips have been developed by our experienced team of cell separation scientists and field specialists, who have worked with some of the top labs worldwide to optimize sample processing and cell separation results.


HIPscience is a collection of recorded scientific presentations and wallcharts/posters covering a variety of topics related to human immunology.


Need help calculating cell recovery after cell separation? Looking for antibodies against human protein targets for your flow cytometry experiments? HIPtools provides user-friendly calculators, public datasets, smartphone apps and other tools for human immunology researchers.


We Want to Hear From You!

Have questions about something on the site? If it’s about a particular protocol or tip, feel free to leave your questions and comments on the individual protocol/tip page. If it’s a more general question or comment, use the contact us page or send us an email directly at

We are always looking for new ideas and tips for HIP. If you know of specific protocols, databases, apps, webcasts or other resources that may be of interest to other human immunology researchers, let us know so that we can share them on HIP.